Analytical Chemistry: Microfluidic sensor for sensing heat-shock mRNA of Cryptosporidium parvum

Esch, M.B.; Locascio, L.; Tarlov, M.J.; Durst, R.A. “Detection of Viable Cryptosporidium parvum in a Microfluidic Chip”, Analytical Chemistry, 2001, 73 (13), 2952-2958.Crypto

In this project we developed a microfluidic RNA sensor that improved sensitivity compared to previously developed injection flow analysis-based sensors 5-fold. The sensor captured RNA from Cryptosporidium parvum within a microfluidic channel. Subsequent flow of oligonucleotide-tagged fluorescent green dye-filled liposomes made the RNA visible within the microfluidic channel. This work was presented at Pittcon in 2001.


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